Anti-Human ATG4D Polyclonal Antibody|抗人ATG4D多克隆抗体(靶标：人自噬相关蛋白4D)
- 货号：RB9THC229014
- 价格：面议
- 点击：359
产品介绍
产品参数
| 形态 | Liquid |
|---|---|
| 宿主种属 | Rabbit |
| 克隆性 | Polyclonal |
| 抗体亚型 | IgG |
| 纯化方式 | Purified by antigen affinity column. |
| 内毒素水平 | Please contact with the lab for this information. |
| 应用 | ELISA, IHC, WB |
| 种属反应性 | Human |
| 靶标 | APG4D, ATG4D, AUT-like 4 cysteine endopeptidase, AUTL4, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D, Cysteine protease ATG4 |
| 免疫原 | E. coli - derived recombinant Human ATG4D (Ser92-Ser420). |
| 储存缓冲液 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
| 产品使用说明 | ELISA:1:5000-1:20000;IHC:1:50-1:500;WB:1:500-1:2000 |
| 登录号 | Q86TL0 |
| 背景信息 | Cysteine protease ATG4D is a ~52 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. 1. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865 2. Kauffman, KJ. et al. (2018) Autophagy 14, 992-1010. PMID: 29458288 3. Agrotis, A. et al. (2019) Autophagy 15, 976-997. PMID: 30661429 4. Durgan, J. et al. (2021) Molecular cell 81, 2031-2040.e8. PMID: 33909989 5. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106 |
| 备注 | For research use only |
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