| ELISA | ELISA | Human | 预测反应 | | |
| Functional Studies (Neut/Block) | FuncSNeutBlock | Human | 保证反应 | | To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1a (20 ng/ml), a concentration of 1.0 - 2.0 μg/ml of this antibody is required. |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Human | 保证反应 | | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 1 µg/mL | Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
| Western blot | WB | Human | 保证反应 | | |
| Sandwich ELISA | sELISA | Human | 保证反应 | | |
| ELISA | ELISA | Recombinant full length protein | 保证反应 | | To detect hIL-1a by direct ELISA (using 100 μl/well antibody solution) a concentration of at least 0.5 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-1a. |
| Functional Studies (Neut/Block) | FuncSNeutBlock | Recombinant full length protein | 不推荐 | | |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Recombinant full length protein | 不推荐 | | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Recombinant full length protein | 不推荐 | | |
| Western blot | WB | Recombinant full length protein | 不推荐 | | |
| Sandwich ELISA | sELISA | Recombinant full length protein | 不推荐 | | |
| ELISA | ELISA | Recombinant full length protein - Human | 不推荐 | | |
| Functional Studies (Neut/Block) | FuncSNeutBlock | Recombinant full length protein - Human | 不推荐 | | |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Recombinant full length protein - Human | 不推荐 | | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Recombinant full length protein - Human | 不推荐 | | |
| Western blot | WB | Recombinant full length protein - Human | 已验证 | | To detect hIL-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich ELISA | sELISA | Recombinant full length protein - Human | 已验证 | 0.5-2 µg/mL | To detect IL-1 αlpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-1 αlpha. |
