犬核因子κB亚基p105(NFKB-p105)酶联免疫吸附检测试剂盒

英文名称	Dog NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
别名	NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B

种属	Dog
检测类型	Sandwich
灵敏度	0.055 ng/mL
检测范围	0.16-10 ng/mL
样本类型	Tissue homogenates, cell lysates and other biological fluids
实验时长	3.5h

研究领域	Signal transduction;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q6F3J0

标准曲线图

【仅供科学研究使用】

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