人热休克蛋白47(HSP47)酶联免疫吸附检测试剂盒

英文名称	Human HSP47(Heat Shock Protein 47) ELISA Kit
别名	CBP2; AsTP3; CBP1; PIG14; RA-A47; SERPINH1; SERPINH2; Colligen; Gp46; Serpin Peptidase Inhibitor Clade H Member 1; Heat Shock Protein 47; Collagen Binding Protein 2

种属	Human
检测类型	Sandwich
灵敏度	51 pg/mL
检测范围	125-8000 pg/mL
样本类型	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
实验时长	3.5h

研究领域	Developmental science;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP47. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP47. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP47, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP47 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P50454

标准曲线图

【仅供科学研究使用】

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