人内皮素1(ET-1)微量上样酶联免疫吸附检测试剂盒

英文名称	Human ET-1(Endothelin1) Microsample ELISA Kit
别名	ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

种属	Human
检测类型	Sandwich
灵敏度	2.69 pg/mL
检测范围	7.82-500 pg/mL
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Endocrinology;Cardiovascular biology;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P05305

标准曲线图

【仅供科学研究使用】

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