人上皮型钙黏蛋白(E-Cad)酶联免疫吸附检测试剂盒

英文名称	Human E-Cad(E-Cadherin) ELISA Kit
别名	CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial

种属	Human
检测类型	Sandwich
灵敏度	0.11 ng/mL
检测范围	0.32-20 ng/mL
样本类型	serum, plasma, urine, tissue homogenates and other biological fluids
实验时长	3.5h

研究领域	CD & Adhesion molecule;Tumor immunity;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P12830

标准曲线图

【仅供科学研究使用】

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