人磷酸二酯酶4B(PDE4B)酶联免疫吸附检测试剂盒

英文名称	Human PDE4B(Phosphodiesterase 4B, cAMP Specific) ELISA Kit
别名	DPDE4; PDE32; Dunce(Drosophila)Homolog Phosphodiesterase E4; cAMP-specific 3',5'-cyclic phosphodiesterase 4B

种属	Human
检测类型	Sandwich
灵敏度	0.063 ng/mL
检测范围	0.16-10 ng/mL
样本类型	Serum, plasma, tissue homogenates and other biological fluids.
实验时长	3.5h

研究领域	Enzyme & Kinase;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDE4B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDE4B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDE4B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDE4B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q07343

标准曲线图

【仅供科学研究使用】

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