人酸性几丁质酶(CHIA)酶联免疫吸附检测试剂盒

英文名称	Human CHIA(Acidic Mammalian Chitinase) ELISA Kit
别名	Acidic mammalian chitinase [Precursor]; Acidic mammalian chitinase; AMCase; Chia; CHIA_HUMAN; CHIT2; chitinase, acidic; DKFZp313J1722; ECF L; Lung specific protein TSA1902; Lung-specific protein TSA1902; RP5 1125M8.1; TSA1902

种属	Human
检测类型	Sandwich
灵敏度	0.073 ng/mL
检测范围	0.16-10 ng/mL
样本类型	serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Immunology; Microbiology; Signal Transduction
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CHIA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CHIA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CHIA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CHIA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q9BZP6

标准曲线图

【仅供科学研究使用】

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