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小鼠固醇调节元件结合转录因子2(SREBP2)酶联免疫吸附检测试剂盒

  • 货号:ELK0001
  • 价格:¥2,800.00 元
  • 点击:7

产品介绍

产品参数

48t1960
96t2800
96t_511900
name_enMouse SREBP2(Sterol Regulatory Element-binding Protein 2) ELISA Kit
alternative_namesSREBP2; SREBP-2; Srebf2_retired
speciesMouse
assay_typeSandwich
sensitivity0.13 ng/mL
standard20 ng/mL
detection_range0.32-20 ng/mL
sample_typeTissue homogenates, cell lysates and other biological fluids
assay_length3.5h
research_areaCardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism
test_principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SREBP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SREBP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SREBP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SREBP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
uniprot_idQ3U1N2
StandardCurveURLhttps://www.elkbiotech.cn/upload/image/ELISA/ELK0001-b-1.jpg
ManualURLhttps://www.elkbiotech.cn/upload/file/ELISA/ELK0001-1.pdf
ProductURLhttps://www.elkbiotech.cn/pro/ELK0001
_product_typeelisa_standard
【仅供科学研究使用】

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