人新型冠状病毒N蛋白酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human SARS-CoV-2 Nucleocapsid ELISA Kit","alternative_names":"COVID-19;SARS-CoV-2; 2019-nCoV","species":"Human","assay_type":"Sandwich","sensitivity":"3.2 pg\/mL","standard":"500 pg\/mL","detection_range":"7.82-500 pg\/mL","sample_type":"serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Infectious Diseases","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SARS-CoV-2 Nucleocapsid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SARS-CoV-2 Nucleocapsid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SARS-CoV-2 Nucleocapsid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SARS-CoV-2 Nucleocapsid in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ECV001-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ECV001-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ECV001",}