| 48t | 1820 |
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| 96t | 2600 |
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| 96t_5 | 11050 |
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| name_en | Human SARS-CoV-2 Nucleocapsid ELISA Kit |
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| alternative_names | COVID-19;SARS-CoV-2; 2019-nCoV |
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| species | Human |
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| assay_type | Sandwich |
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| sensitivity | 3.2 pg/mL |
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| standard | 500 pg/mL |
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| detection_range | 7.82-500 pg/mL |
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| sample_type | serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| assay_length | 3.5h |
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| research_area | Infectious Diseases |
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| test_principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SARS-CoV-2 Nucleocapsid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SARS-CoV-2 Nucleocapsid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SARS-CoV-2 Nucleocapsid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SARS-CoV-2 Nucleocapsid in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| StandardCurveURL | https://www.elkbiotech.cn/upload/image/ELISA/ECV001-b-1.jpg |
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| ManualURL | https://www.elkbiotech.cn/upload/file/ELISA/ECV001-1.pdf |
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| ProductURL | https://www.elkbiotech.cn/pro/ECV001 |
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| _product_type | elisa_standard |
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