Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed

中文名称	山羊抗鸡IgY H&L (Alexa Fluor® 405)预吸附二抗
别名	Ig gamma 1 chain C region、Ig gamma 3 chain C region、Ig gamma 4 chain C region、Ig gamma-2 chain C region、Immunoglobin heavy constant gamma 1、Immunoglobulin G
靶点符号	Chicken
产品类型	二抗

宿主种属	Goat
克隆	Polyclonal
亚型	IgG
偶联物	Alexa Fluor® 405
激发波长/发射波长	Ex: 401nm, Em: 421nm
反应种属	Chicken
应用	ICC/IF、IHC-P、IHC-Fr、ELISA、Flow Cyt
靶标种属	Chicken
靶标亚型	IgY
靶标特异性	Heavy & Light chains
预吸附	Yes
最低交叉反应	Mouse, Rat, Rabbit, Horse, Cow, Human, Pig
特异性	By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
浓度	2 mg/mL

应用	验证状态	推荐稀释	备注
ELISA	已验证
Flow Cyt	已验证	1/2000
ICC/IF	已验证	1/200 - 1/1000	We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor ® 405 dye (see difference below) Ex max: Alexa Fluor ® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor ® 405 = 421nm / DAPI = 461nm
IHC-Fr	已验证
IHC-P	已验证

形式	Liquid
纯化工艺	Affinity purification Immunogen
纯化说明	Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. The antibody to chicken IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
存储溶液	Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
推荐的短期储存时间	1-2 weeks
储存信息	Avoid freeze / thaw cycle\|Stable for 12 months at -20°C\|Store in the dark

【仅供科学研究使用】

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