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Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed

  • 货号:RB9A37060967
  • 价格:面议
  • 点击:1455
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产品介绍

产品描述

别名Ig gamma 1 chain C region、Ig gamma 3 chain C region、Ig gamma 4 chain C region、Ig gamma-2 chain C region、Immunoglobin heavy constant gamma 1、Immunoglobulin G
靶点符号Goat
产品类型二抗

关键信息

宿主种属Donkey
克隆Polyclonal
亚型IgG
偶联物Alexa Fluor® 405
激发波长/发射波长Ex: 401nm, Em: 421nm
反应种属Goat
应用IHC-P、ICC/IF、IHC-Fr、ELISA、Flow Cyt
靶标种属Goat
靶标亚型IgG
靶标特异性Heavy & Light chains
预吸附Yes
最低交叉反应Mouse, Rat, Rabbit, Chicken, Human, Pig
免疫原The exact immunogen used to generate this antibody is proprietary information.
特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
浓度2 mg/mL

反应性数据

应用验证状态推荐稀释备注
ELISA已验证
Flow Cyt已验证1/2000
ICC/IF已验证1/200 - 1/1000We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor ® 405 dye (see difference below) Ex max: Alexa Fluor ® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor ® 405 = 421nm / DAPI = 461nm
IHC-Fr已验证
IHC-P已验证

储存与纯化

形式Liquid
纯化工艺Affinity purification Immunogen
纯化说明Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
存储溶液Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
推荐的短期储存时间1-2 weeks
储存信息Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
【仅供科学研究使用】

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