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小鼠IgG用于免疫沉淀(Anti-mouse IgG for IP (Biotin))

  • 货号:RB9A37060787
  • 价格:面议
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产品介绍

产品描述

中文名称抗小鼠IgG for IP (Biotin)
英文名称Anti-mouse IgG for IP (Biotin)
别名Igh-4、Ighg1、Ig gamma-1 chain C region secreted form、Igh-4、Ighg1
靶点符号Ighg1
产品类型二抗

关键信息

宿主种属Rat
克隆Monoclonal
亚型IgG
偶联物Biotin
反应种属Mouse
应用WB
靶标种属Mouse
靶标亚型IgG
靶标特异性Heavy & Light chains
预吸附No
特异性VeriBlot for IP secondary antibodies are anti-mouse immunoblotting reagents that enable the trouble-free detection of immunoblotted target protein bands, without interference from denatured IgG. This allows to detect the (co-)immunoprecipitated protein without masking by the IgG heavy (50 kDa) and light chains (25 kDa). In general this interference tends to originate from secondary antibodies which recognize primary antibodies released with the antigen during the immunoprecipitation procedure or endogenous IgGs from the lysate itself. VeriBlot for IP secondary antibodies only recognize native (non-reduced) antibodies and therefore the detection of heavy and light chains is highly minimized, if the immunoprecipitate is fully reduced.
浓度0.5 mg/mL

反应性数据

应用验证状态推荐稀释备注
WB已验证0.5 µg/mLThe dilution will depend on the sensitivity of the avidin/streptavidin HRP. The recommended is dilution is 0.5 µg/ml. Based on a 0.5 µg/ml dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples in combination with avidin/streptavidin HRP.

储存与纯化

形式Liquid
存储溶液Preservative: 0.09% Sodium azide Constituents: 0.88% Sodium chloride, 0.16% Sodium phosphate
推荐的短期储存时间1-2 weeks
储存信息Avoid freeze / thaw cycle
【仅供科学研究使用】

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