抗MYL12A抗体（Anti-MYL12A (phospho S19) antibody）

货号：RB9A37028106
价格：面议
点击：1367

产品介绍

产品描述

中文名称	Anti-MYL12A (phospho S19)抗体
英文名称	Anti-MYL12A (phospho S19) antibody
别名	MLCB、MRLC3、RLC、MYL12A、Myosin regulatory light chain 12A、Epididymis secretory protein Li 24、MLC-2B、Myosin RLC、Myosin regulatory light chain MRLC3、HEL-S-24
靶点符号	MYL12A
产品类型	一抗

关键信息

宿主种属	Rabbit
克隆	Polyclonal
亚型	IgG
不含载体蛋白	No
反应种属	Human、Mouse、Caenorhabditis elegans、Rat
应用	IHC-P、WB、IHC-FoFr、ELISA、ICC/IF、IP
免疫原	Synthetic Peptide within Human MYL12A pS19 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.；P19105
特异性	RB9A37028106 can detect the phosphorylated serine residue in both the human and mouse protein. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.This phospho specific polyclonal antibody is specific for phosphorylated pS19 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.Customers have seen variable performance with Xenopus laevis.
浓度	1.19 mg/mL

反应性数据

应用	应用代码	物种	验证状态	推荐稀释	备注
ELISA	ELISA	Bird	预测反应
Immunocytochemistry/ Immunofluorescence	ICCIF	Bird	预测反应
Immunohistochemistry (PFA perfusion fixed frozen sections)	IHCFoFr	Bird	预测反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)	IHCP	Bird	预测反应
Immunoprecipitation	IP	Bird	预测反应
Western blot	WB	Bird	预测反应
ELISA	ELISA	Caenorhabditis elegans	保证反应	1/10000 - 1/30000
Immunocytochemistry/ Immunofluorescence	ICCIF	Caenorhabditis elegans	保证反应
Immunohistochemistry (PFA perfusion fixed frozen sections)	IHCFoFr	Caenorhabditis elegans	保证反应		Trichloroacetic acid was used for tissue fixation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)	IHCP	Caenorhabditis elegans	预测反应
Immunoprecipitation	IP	Caenorhabditis elegans	保证反应
Western blot	WB	Caenorhabditis elegans	预测反应
ELISA	ELISA	Human	保证反应	1/10000 - 1/30000
Immunocytochemistry/ Immunofluorescence	ICCIF	Human	保证反应
Immunohistochemistry (PFA perfusion fixed frozen sections)	IHCFoFr	Human	保证反应		Trichloroacetic acid was used for tissue fixation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)	IHCP	Human	已验证	2.5 µg/mL
Immunoprecipitation	IP	Human	保证反应
Western blot	WB	Human	保证反应
ELISA	ELISA	Mammals	预测反应
Immunocytochemistry/ Immunofluorescence	ICCIF	Mammals	预测反应
Immunohistochemistry (PFA perfusion fixed frozen sections)	IHCFoFr	Mammals	预测反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)	IHCP	Mammals	预测反应
Immunoprecipitation	IP	Mammals	预测反应
Western blot	WB	Mammals	预测反应
ELISA	ELISA	Mouse	保证反应	1/10000 - 1/30000
Immunocytochemistry/ Immunofluorescence	ICCIF	Mouse	保证反应
Immunohistochemistry (PFA perfusion fixed frozen sections)	IHCFoFr	Mouse	保证反应		Trichloroacetic acid was used for tissue fixation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)	IHCP	Mouse	保证反应
Immunoprecipitation	IP	Mouse	保证反应
Western blot	WB	Mouse	已验证	1/500 - 1/2000	We recommend using BSA for blocking when using phospho-specific antibodies.
ELISA	ELISA	Rat	保证反应	1/10000 - 1/30000
Immunocytochemistry/ Immunofluorescence	ICCIF	Rat	保证反应
Immunohistochemistry (PFA perfusion fixed frozen sections)	IHCFoFr	Rat	保证反应		Trichloroacetic acid was used for tissue fixation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)	IHCP	Rat	预测反应
Immunoprecipitation	IP	Rat	保证反应
Western blot	WB	Rat	预测反应
ELISA	ELISA	Recombinant fragment	保证反应	1/10000 - 1/30000
Immunocytochemistry/ Immunofluorescence	ICCIF	Recombinant fragment	不推荐
Immunohistochemistry (PFA perfusion fixed frozen sections)	IHCFoFr	Recombinant fragment	不推荐
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)	IHCP	Recombinant fragment	不推荐
Immunoprecipitation	IP	Recombinant fragment	保证反应
Western blot	WB	Recombinant fragment	不推荐
ELISA	ELISA	Recombinant full length protein	不推荐
Immunocytochemistry/ Immunofluorescence	ICCIF	Recombinant full length protein	不推荐
Immunohistochemistry (PFA perfusion fixed frozen sections)	IHCFoFr	Recombinant full length protein	不推荐
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)	IHCP	Recombinant full length protein	不推荐
Immunoprecipitation	IP	Recombinant full length protein	不推荐
Western blot	WB	Recombinant full length protein	已验证	1/500 - 1/2000	We recommend using BSA for blocking when using phospho-specific antibodies.

储存与纯化

形式	Liquid
纯化工艺	Affinity purification Immunogen
纯化说明	This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
存储溶液	pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
推荐的短期储存时间	1-2 weeks
储存信息	Avoid freeze / thaw cycle

【仅供科学研究使用】

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