| Immunocytochemistry/ Immunofluorescence | ICCIF | Human | 已验证 | | Fix cells with 4% paraformaldehyde in NWB (200 mM sucrose, 15 mM Hepes, pH 7.4, 50 mM NaCl, 2.5 mM MgCl2, and 1 mM DTT). Permeabilise with 0.1% NP-40 or 0.1% Triton X-100 in PBS for 2 min. (see Lopez-Soler reference); different customer have used this antibody at different dilutions for ICC/IF (see images below). We recommend that optimal working dilutions are determined by each customer. |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 0.5-1 µg/mL | |
| Western blot | WB | Human | 已验证 | 1/2000 - 1/5000 | |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Mouse | 已验证 | | Fix cells with 4% paraformaldehyde in NWB (200 mM sucrose, 15 mM Hepes, pH 7.4, 50 mM NaCl, 2.5 mM MgCl2, and 1 mM DTT). Permeabilise with 0.1% NP-40 or 0.1% Triton X-100 in PBS for 2 min. (see Lopez-Soler reference); different customer have used this antibody at different dilutions for ICC/IF (see images below). We recommend that optimal working dilutions are determined by each customer. |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Mouse | 保证反应 | | |
| Western blot | WB | Mouse | 已验证 | 1/2000 - 1/5000 | |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Rat | 保证反应 | | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Rat | 保证反应 | | |
| Western blot | WB | Rat | 已验证 | 1/2000 - 1/5000 | |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Saccharomyces cerevisiae | 已验证 | | Fix cells with 4% paraformaldehyde in NWB (200 mM sucrose, 15 mM Hepes, pH 7.4, 50 mM NaCl, 2.5 mM MgCl2, and 1 mM DTT). Permeabilise with 0.1% NP-40 or 0.1% Triton X-100 in PBS for 2 min. (see Lopez-Soler reference); different customer have used this antibody at different dilutions for ICC/IF (see images below). We recommend that optimal working dilutions are determined by each customer. |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Saccharomyces cerevisiae | 保证反应 | | |
| Western blot | WB | Saccharomyces cerevisiae | 保证反应 | | |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Vertebrata | 预测反应 | | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Vertebrata | 预测反应 | | |
| Western blot | WB | Vertebrata | 预测反应 | | |
