抗HIF-1α抗体（Anti-HIF-1 alpha antibody (RB9A37026061)）
- 货号：RB9A37026061
- 价格：面议
- 点击：1696
产品介绍
产品描述
| 中文名称 | 重组Anti-HIF-1 alpha抗体（RB9A37026061） |
|---|---|
| 英文名称 | Anti-HIF-1 alpha antibody (RB9A37026061) |
| 别名 | BHLHE78、MOP1、PASD8、HIF1A、Hypoxia-inducible factor 1-alpha、HIF-1-alpha、HIF1-alpha、ARNT-interacting protein、Basic-helix-loop-helix-PAS protein MOP1、Class E basic helix-loop-helix protein 78、Member of PAS protein 1、PAS domain-containing protein 8、bHLHe78 |
| 靶点符号 | HIF1A |
| 产品类型 | 一抗 |
关键信息
| 宿主种属 | Rabbit |
|---|---|
| 克隆 | Monoclonal |
| 亚型 | IgG |
| 不含载体蛋白 | No |
| 反应种属 | Human |
| 应用 | ChIP-seq、IP、WB、IHC-P、ICC |
| 免疫原 | Recombinant Fragment Protein within Human HIF1A aa 400-850. The exact immunogen used to generate this antibody is proprietary information.；Q16665 |
反应性数据
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| ChIP-sequencing | ChIPseq | Human | 已验证 | 10 µL for 30 µL chromatin | |
| Immunocytochemistry | ICC | Human | 已验证 | 1/100 - 1/1000 | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 1/100 - 1/1000 | Antigen retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
| Immunoprecipitation | IP | Human | 已验证 | Use 10μl/0.5mg lysate. | |
| Western blot | WB | Human | 已验证 | 1/1000 | Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate. Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate . For a stronger signal, HeLa-DFO treated nuclear extracts are recommended . The cell fractionation kit can also be purchased separately . Negative Control: Most normal tissues or cells, other than kidney or heart. The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below: 40-80 kDa - degraded; 10-130 kDa - post translationally modified; ~200 kDa - heterodimer with HIF-1 beta |
储存与纯化
| 形式 | Liquid |
|---|---|
| 纯化说明 | Recombinant antibody was purified from cell culture supernatant. |
| 存储溶液 | pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA |
| 推荐的短期储存时间 | 1-2 weeks |
| 储存信息 | Avoid freeze / thaw cycle |
相关产品:
