| ELISA | ELISA | Human | 保证反应 | |
| Flow Cytometry (Intracellular) | FlowCytIntra | Human | 已验证 | RB9A37012990 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Human | 已验证 | |
| Western blot | WB | Human | 已验证 | Induction of cells with Pervanadate: Prepare 500mM stock Sodium Orthovanadate in PBS. To make 100mM (100x) Pervanadate Na3VO4 right before experiment, add 400ul 500mM Sodium Orthovanadate to 1560ul H2O and then 40ul H2O2, vortex and incubate at room RT for 5-10min. Cells are serum-starved overnight, and then treated with freshly made Pervanadate (100x, final concentration 1mM) for 20min at 37°C. |
| ELISA | ELISA | Mouse | 预测反应 | |
| Flow Cytometry (Intracellular) | FlowCytIntra | Mouse | 保证反应 | RB9A37007757 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Mouse | 保证反应 | |
| Western blot | WB | Mouse | 保证反应 | Induction of cells with Pervanadate: Prepare 500mM stock Sodium Orthovanadate in PBS. To make 100mM (100x) Pervanadate Na3VO4 right before experiment, add 400ul 500mM Sodium Orthovanadate to 1560ul H2O and then 40ul H2O2, vortex and incubate at room RT for 5-10min. Cells are serum-starved overnight, and then treated with freshly made Pervanadate (100x, final concentration 1mM) for 20min at 37°C. |
| ELISA | ELISA | Rat | 保证反应 | |
| Flow Cytometry (Intracellular) | FlowCytIntra | Rat | 保证反应 | RB9A37007757 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Rat | 已验证 | |
| Western blot | WB | Rat | 已验证 | Induction of cells with Pervanadate: Prepare 500mM stock Sodium Orthovanadate in PBS. To make 100mM (100x) Pervanadate Na3VO4 right before experiment, add 400ul 500mM Sodium Orthovanadate to 1560ul H2O and then 40ul H2O2, vortex and incubate at room RT for 5-10min. Cells are serum-starved overnight, and then treated with freshly made Pervanadate (100x, final concentration 1mM) for 20min at 37°C. |
| ELISA | ELISA | Recombinant fragment - Human | 已验证 | |
| Flow Cytometry (Intracellular) | FlowCytIntra | Recombinant fragment - Human | 不推荐 | |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Recombinant fragment - Human | 不推荐 | |
| Western blot | WB | Recombinant fragment - Human | 不推荐 | |
