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抗p53抗体(Anti-p53 antibody - BSA and Azide free [Mouse])

  • 货号:RB9A37023440
  • 价格:面议
  • 点击:2005
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产品介绍

产品描述

中文名称Anti-p53抗体 - BSA and Azide free(RB9A37023440)
英文名称Anti-p53 antibody - BSA and Azide free [Mouse]
别名P53、TP53、Cellular tumor antigen p53、Antigen NY-CO-13、Phosphoprotein p53、Tumor suppressor p53
靶点符号TP53
产品类型一抗

关键信息

宿主种属Mouse
克隆Monoclonal
亚型IgG2a
轻链类型kappa
不含载体蛋白Yes
反应种属Human
应用WB、Flow Cyt (Intra)、IHC-P、ICC/IF、ChIP
免疫原The exact immunogen used to generate this antibody is proprietary information.
特异性This antibody clone recognises both wild-type and mutant forms of p53 in human samples. It is not designed to recognise any specific p53 mutation.We have confirmed this experimentally and have been able to detect p53 in different cell lines using various applications and treatments.Important note: p53 expression levels vary greatly between cell lines. It has been reported that p53 mutations render the protein more stable, hence mutated cell lines often express higher levels of the p53 protein compared to wild-type cell lines. For low expressing wild type cell lines, p53 expression can be increased with cell treatments such as camptothecin or irinotecan.
表位The epitope maps to within aa 20-25.

反应性数据

应用应用代码物种验证状态推荐稀释备注
ChIPChIPHuman保证反应
Flow Cytometry (Intracellular)FlowCytIntraHuman已验证
Immunocytochemistry/ ImmunofluorescenceICCIFHuman已验证
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPHuman已验证1-5 µg/mLPerform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blotWBHuman已验证1/1000
ChIPChIPMouse不推荐
Flow Cytometry (Intracellular)FlowCytIntraMouse不推荐
Immunocytochemistry/ ImmunofluorescenceICCIFMouse不推荐
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPMouse不推荐1-5 µg/mLPerform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blotWBMouse不推荐1/1000
ChIPChIPRat不推荐
Flow Cytometry (Intracellular)FlowCytIntraRat不推荐
Immunocytochemistry/ ImmunofluorescenceICCIFRat不推荐
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPRat不推荐1-5 µg/mLPerform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blotWBRat不推荐1/1000

储存与纯化

形式Liquid
存储溶液Constituents: PBS
储存信息Do Not Freeze
【仅供科学研究使用】

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