| Immunohistochemistry (Frozen sections) | IHCFr | Human | 保证反应 | | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 1/500 | Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
| Immunoprecipitation | IP | Human | 保证反应 | | |
| Western blot | WB | Human | 已验证 | 1/1000 | |
| Immunohistochemistry (Frozen sections) | IHCFr | Mouse | 已验证 | 1/500 | Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Mouse | 已验证 | 1/500 | Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
| Immunoprecipitation | IP | Mouse | 已验证 | 1/30 | |
| Western blot | WB | Mouse | 已验证 | 1/1000 | In Western blot analysis, RB9A37001182 exhibited lower sensitivity compared to RB9A37000749 and RB9A37021708, for which reason we recommend the latter two as superior alternatives. Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1:500), and fg-grade ECL substrates are suggested if RB9A37001182 must be used. |
| Immunohistochemistry (Frozen sections) | IHCFr | Rat | 已验证 | 1/500 | Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Rat | 已验证 | 1/500 | Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
| Immunoprecipitation | IP | Rat | 保证反应 | | |
| Western blot | WB | Rat | 已验证 | 1/1000 | In Western blot analysis, RB9A37001182 exhibited lower sensitivity compared to RB9A37000749 and RB9A37021708, for which reason we recommend the latter two as superior alternatives. Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1:500), and fg-grade ECL substrates are suggested if RB9A37001182 must be used. |
