| 中文名称 | Anti-Piccolo抗体(RB9A37014652) |
|---|---|
| 别名 | ACZ、KIAA0559、PCLO、Protein piccolo、Aczonin |
| 靶点符号 | PCLO |
| 产品类型 | 一抗 |
| 宿主种属 | Rabbit |
|---|---|
| 克隆 | Polyclonal |
| 亚型 | IgG |
| 不含载体蛋白 | No |
| 反应种属 | Mouse、Rat、Human |
| 应用 | IHC (PFA fixed)、WB、IHC-P |
| 免疫原 | The exact immunogen used to generate this antibody is proprietary information. |
| 特异性 | Due to its large size, Piccolo requires special gel-electrophoresis and Western blotting protocols for visualization by immunoblotting. Excellent results can be obtained, for example, with the 4-12% TRIS-glycine gradient gels of Anamed. For success in WB with Rabbit polyclonal to Piccolo - Synaptic Marker (RB9A37014652)), do not denature WB sample lysate. |
| 浓度 | 0.36 mg/mL |
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 保证反应 | 5 µg/mL | Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
| Immunohistochemistry (PFA fixed) | IHCPFAfixed | Human | 保证反应 | 1/300 - 1/1000 | |
| Western blot | WB | Human | 已验证 | 0.2-0.5 µg/mL | Abcam recommends using milk as the blocking agent. |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Mouse | 保证反应 | 5 µg/mL | Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
| Immunohistochemistry (PFA fixed) | IHCPFAfixed | Mouse | 保证反应 | 1/300 - 1/1000 | |
| Western blot | WB | Mouse | 已验证 | 0.2-0.5 µg/mL | Abcam recommends using milk as the blocking agent. |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Rat | 已验证 | 5 µg/mL | Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
| Immunohistochemistry (PFA fixed) | IHCPFAfixed | Rat | 已验证 | 1/300 - 1/1000 | |
| Western blot | WB | Rat | 已验证 | 0.2-0.5 µg/mL | Abcam recommends using milk as the blocking agent. |
| 形式 | Liquid |
|---|---|
| 纯化工艺 | Affinity purification Immunogen |
| 存储溶液 | pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA |
| 推荐的短期储存时间 | 1-2 weeks |
| 储存信息 | Avoid freeze / thaw cycle |