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抗白细胞介素1β抗体(Anti-IL-1 beta antibody [Rabbit])

  • 货号:RB9A37014489
  • 价格:面议
  • 点击:1594
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产品介绍

产品描述

中文名称Anti-IL-1 beta抗体(RB9A37014489)
英文名称Anti-IL-1 beta antibody [Rabbit]
别名Interleukin-1 beta、IL-1 beta、Il1b
靶点符号Il1b
产品类型一抗

关键信息

宿主种属Rabbit
克隆Polyclonal
亚型IgG
不含载体蛋白No
反应种属Mouse
应用WB、IHC-P
免疫原Recombinant Full Length Protein corresponding to Mouse Il1b.;P10749
特异性RB9A37014489 is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1 beta or mouse IL1 alpha based on a neutralization assay. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1 beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL-1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody.
浓度1 mg/mL

反应性数据

应用应用代码物种验证状态推荐稀释备注
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPMouse已验证2 µg/mLPerform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blotWBMouse已验证

储存与纯化

形式Liquid
纯化工艺Ion exchange chromatography DEAE-C
纯化说明This is an IgG preparation of whole rabbit serum purified by DEAE fractionation.
存储溶液Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
推荐的短期储存时间1-2 weeks
储存信息Avoid freeze / thaw cycle
【仅供科学研究使用】

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