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F-肌动蛋白抗体(Anti-F-actin antibody)

  • 货号:RB9A37014331
  • 价格:面议
  • 点击:1958
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产品介绍

产品描述

中文名称Anti-F-actin 抗体
英文名称Anti-F-actin antibody
别名Beta-actin、ACTB
靶点符号ACTB
产品类型一抗

关键信息

宿主种属Mouse
克隆Monoclonal
亚型IgM
轻链类型unknown
不含载体蛋白No
反应种属Rat、Rabbit、Human、Mouse
应用Flow Cyt、IHC-R、IHC-Fr、WB、ELISA、ICC/IF
特异性recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
浓度1 mg/mL
表位This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.

反应性数据

应用应用代码物种验证状态推荐稀释备注
ELISAELISAHuman保证反应
Flow CytometryFlowCytHuman保证反应- Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFHuman保证反应
Immunohistochemistry (Frozen sections)IHCFrHuman保证反应
Immunohistochemistry (Resin sections)IHCRHuman保证反应
Western blotWBHuman已验证1/100 - 1/500As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
ELISAELISAMouse保证反应
Flow CytometryFlowCytMouse保证反应- Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFMouse保证反应
Immunohistochemistry (Frozen sections)IHCFrMouse保证反应
Immunohistochemistry (Resin sections)IHCRMouse保证反应
Western blotWBMouse保证反应1/100 - 1/500As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
ELISAELISARabbit保证反应
Flow CytometryFlowCytRabbit保证反应- Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFRabbit保证反应
Immunohistochemistry (Frozen sections)IHCFrRabbit保证反应
Immunohistochemistry (Resin sections)IHCRRabbit保证反应
Western blotWBRabbit保证反应1/100 - 1/500As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
ELISAELISARat保证反应
Flow CytometryFlowCytRat保证反应- Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFRat保证反应
Immunohistochemistry (Frozen sections)IHCFrRat保证反应
Immunohistochemistry (Resin sections)IHCRRat保证反应
Western blotWBRat保证反应1/100 - 1/500As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.

储存与纯化

形式Liquid
纯化说明Purified IgM prepared from tissue culture supernatant.
存储溶液Preservative: 0.1% Sodium azide
推荐的短期储存时间1-2 weeks
储存信息Avoid freeze / thaw cycle
纯度IgM fraction
【仅供科学研究使用】

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