HRP Anti-p53 antibody
- 货号：RB9A37014218
- 价格：面议
- 点击：2051
产品介绍
产品描述
| 中文名称 | HRP Anti-p53抗体 |
|---|---|
| 别名 | P53、TP53、Cellular tumor antigen p53、Antigen NY-CO-13、Phosphoprotein p53、Tumor suppressor p53 |
| 靶点符号 | TP53 |
| 产品类型 | 一抗 |
关键信息
| 宿主种属 | Mouse |
|---|---|
| 克隆 | Monoclonal |
| 亚型 | IgG2a |
| 轻链类型 | kappa |
| 偶联物 | HRP |
| 不含载体蛋白 | No |
| 反应种属 | Human |
| 应用 | WB |
| 免疫原 | The exact immunogen used to generate this antibody is proprietary information. |
| 特异性 | This antibody clone recognises both wild-type and mutant forms of p53 in human samples. It is not designed to recognise any specific p53 mutation.We have confirmed this experimentally and have been able to detect p53 in different cell lines using various applications and treatments.Important note: p53 expression levels vary greatly between cell lines. It has been reported that p53 mutations render the protein more stable, hence mutated cell lines often express higher levels of the p53 protein compared to wild-type cell lines. For low expressing wild type cell lines, p53 expression can be increased with cell treatments such as camptothecin or irinotecan. |
| 浓度 | 0.5 mg/mL |
| 表位 | RB9A37014218 recognizes an epitope within aa 20-25 at the N-terminus of Human p53. |
反应性数据
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 |
|---|---|---|---|---|
| Western blot | WB | Human | 已验证 | 1/5000 |
储存与纯化
| 形式 | Liquid |
|---|---|
| 纯化工艺 | Affinity purification Immunogen |
| 存储溶液 | pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA |
| 推荐的短期储存时间 | 1-2 weeks |
| 储存信息 | Avoid freeze / thaw cycle|Store in the dark |
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