| 中文名称 | Anti-Vimentin 抗体(RB9A37013953) |
|---|---|
| 英文名称 | Anti-Vimentin antibody [Mouse] |
| 别名 | Vimentin、VIM |
| 靶点符号 | VIM |
| 产品类型 | 一抗 |
| 宿主种属 | Mouse |
|---|---|
| 克隆 | Monoclonal |
| 亚型 | IgM |
| 轻链类型 | unknown |
| 不含载体蛋白 | No |
| 反应种属 | Mouse、Rat、Human、Chicken |
| 应用 | WB、Flow Cyt、ICC/IF、IHC-P |
| 免疫原 | The exact immunogen used to generate this antibody is proprietary information. |
| 特异性 | The antibody reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types. |
| 浓度 | 1 mg/mL |
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| Flow Cytometry | FlowCyt | Chicken | 保证反应 | ||
| Immunocytochemistry/ Immunofluorescence | ICCIF | Chicken | 已验证 | 1 µg/mL | Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Chicken | 保证反应 | ||
| Western blot | WB | Chicken | 保证反应 | ||
| Flow Cytometry | FlowCyt | Human | 保证反应 | ||
| Immunocytochemistry/ Immunofluorescence | ICCIF | Human | 已验证 | 1 µg/mL | Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 1-5 µg/mL | |
| Western blot | WB | Human | 已验证 | ||
| Flow Cytometry | FlowCyt | Mouse | 已验证 | 1 µg for 10^6 Cells | - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Mouse | 保证反应 | ||
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Mouse | 保证反应 | ||
| Western blot | WB | Mouse | 保证反应 | ||
| Flow Cytometry | FlowCyt | Pig | 预测反应 | ||
| Immunocytochemistry/ Immunofluorescence | ICCIF | Pig | 预测反应 | ||
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Pig | 预测反应 | ||
| Western blot | WB | Pig | 预测反应 | ||
| Flow Cytometry | FlowCyt | Rat | 保证反应 | ||
| Immunocytochemistry/ Immunofluorescence | ICCIF | Rat | 已验证 | 1 µg/mL | Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Rat | 保证反应 | ||
| Western blot | WB | Rat | 保证反应 |
| 形式 | Liquid |
|---|---|
| 纯化工艺 | Proprietary technique |
| 纯化说明 | Purified by precipitation and chromatography. |
| 存储溶液 | pH: 8 Preservative: 0.097% Sodium azide Constituents: PBS |
| 推荐的短期储存时间 | 1-2 weeks |
| 储存信息 | Avoid freeze / thaw cycle |