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抗Nectin-4抗体（Anti-Nectin-4 antibody [Rabbit]）
- 货号：RB9A37011376
- 价格：面议
- 点击：1140
产品介绍
产品描述
| 中文名称 | 重组Anti-Nectin-4抗体（RB9A37011376） |
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| 英文名称 | Anti-Nectin-4 antibody [Rabbit] |
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| 别名 | LNIR、PRR4、PVRL4、NECTIN4、Nectin-4、Ig superfamily receptor LNIR、Nectin cell adhesion molecule 4、Poliovirus receptor-related protein 4 |
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| 靶点符号 | NECTIN4 |
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| 产品类型 | 一抗 |
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关键信息
| 宿主种属 | Rabbit |
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| 克隆 | Monoclonal |
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| 亚型 | IgG |
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| 不含载体蛋白 | No |
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| 反应种属 | Human |
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| 应用 | WB、IHC-P |
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| 免疫原 | The exact immunogen used to generate this antibody is proprietary information. |
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| 特异性 | Customers should note, the current antibody has been examined with alternative automated IHC staining platforms and optimal results were obtained using the Ventana DISCOVERY ULTRA with associated kits and reagents. We were not able to obtain optimal results with Leica BOND platform when using the Polymer Refine detection kit, 20min ER1 antigen retrieval and 16min antibody incubation at room temperature. Further optimisation may be needed when using the Leica BOND platform.The antibody detected a 50kDa and 70kDa non-specific band in the negative sample MDA-MB-231, which are close to the target band. Therefore, we recommend including a negative sample when performing Western Blot analysis.We suggest using the whole cell and cell supernatant lysate side by side to observe the expression of different form of Nectin-4. The cell supernatant can detect soluble Nectin-4 mainly (PMID: 15784625).Negative control: MDA-MB-231 (15784625, PMID: 31617074, PMID: 24386110, PMID: 17474988). |
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| 浓度 | 1.41 mg/mL |
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储存与纯化
| 形式 | Liquid |
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| 纯化工艺 | Affinity purification Protein A |
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| 存储溶液 | pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA |
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| 推荐的短期储存时间 | 1-2 weeks |
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| 储存信息 | Avoid freeze / thaw cycle |
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