抗血小板内皮细胞黏附分子1抗体（Anti-PECAM-1/CD31 antibody (RB9A37010414)）
- 货号：RB9A37010414
- 价格：面议
- 点击：1723
产品介绍
产品描述
| 中文名称 | Anti-PECAM-1/CD31抗体（RB9A37010414） |
|---|---|
| 英文名称 | Anti-PECAM-1/CD31 antibody (RB9A37010414) |
| 别名 | CD31、Platelet endothelial cell adhesion molecule、PECAM-1、EndoCAM、GPIIA'、PECA1、PECAM1 |
| 靶点符号 | PECAM-1 |
| 产品类型 | 一抗 |
关键信息
| 宿主种属 | Mouse |
|---|---|
| 克隆 | Monoclonal |
| 亚型 | IgG1 |
| 轻链类型 | kappa |
| 不含载体蛋白 | No |
| 反应种属 | Human |
| 应用 | IHC-P、Flow Cyt、WB、ICC |
| 免疫原 | Recombinant Full Length Protein corresponding to Human PECAM-1/CD31.；P16284 |
| 浓度 | 0.2 mg/mL |
反应性数据
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| Flow Cytometry | FlowCyt | Human | 已验证 | 1-2 µg for 10^6 Cells | RB9A37007391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Immunocytochemistry | ICC | Human | 已验证 | 1-2 µg/mL | Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 1-2 µg/mL | The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
| Western blot | WB | Human | 已验证 | 1-2 µg/mL | Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Hu Isoform 1-6: 79-83 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates ; Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate . |
储存与纯化
| 形式 | Liquid |
|---|---|
| 纯化工艺 | Affinity purification Protein G |
| 存储溶液 | pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA |
| 推荐的短期储存时间 | 1-2 weeks |
| 储存信息 | Avoid freeze / thaw cycle |
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