| 中文名称 | 重组Anti-MLKL (phospho S358)抗体 |
|---|---|
| 英文名称 | Anti-MLKL (phospho S358) antibody |
| 别名 | Mixed lineage kinase domain-like protein、hMLKL、MLKL |
| 靶点符号 | MLKL |
| 产品类型 | 一抗 |
| 宿主种属 | Rabbit |
|---|---|
| 克隆 | Monoclonal |
| 亚型 | IgG |
| 不含载体蛋白 | No |
| 反应种属 | Human |
| 应用 | WB、Dot、IHC-P |
| 免疫原 | The exact immunogen used to generate this antibody is proprietary information. |
| 特异性 | Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the product protocols section. This file includes key technical notes of experience when using this product. |
| 浓度 | 0.855 mg/mL |
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| Dot Blot | Dot | Human | 保证反应 | ||
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 1/250 - 1/500 | Not Suitable for Mouse and Rat Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Western blot | WB | Human | 已验证 | 1/1000 - 1/2000 | We recommend using 1% SDS Hot lysis method to prepare cell lysates. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here. |
| Dot Blot | Dot | Mouse | 不推荐 | 1/1000 | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Mouse | 不推荐 | 1/250 - 1/500 | Not Suitable for Mouse and Rat Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Western blot | WB | Mouse | 不推荐 | 1/1000 - 1/2000 | We recommend using 1% SDS Hot lysis method to prepare cell lysates. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here. |
| Dot Blot | Dot | Rat | 不推荐 | 1/1000 | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Rat | 不推荐 | 1/250 - 1/500 | Not Suitable for Mouse and Rat Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Western blot | WB | Rat | 不推荐 | 1/1000 - 1/2000 | We recommend using 1% SDS Hot lysis method to prepare cell lysates. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here. |
| Dot Blot | Dot | Synthetic peptide - Human | 已验证 | 1/1000 | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Synthetic peptide - Human | 不推荐 | ||
| Western blot | WB | Synthetic peptide - Human | 不推荐 |
| 形式 | Liquid |
|---|---|
| 纯化工艺 | Affinity purification Protein A |
| 存储溶液 | pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA |
| 推荐的短期储存时间 | 1-2 weeks |
| 储存信息 | Avoid freeze / thaw cycle |