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抗MLKL抗体(Anti-MLKL (phospho S358) antibody)

  • 货号:RB9A37010341
  • 价格:面议
  • 点击:1898
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产品介绍

产品描述

中文名称重组Anti-MLKL (phospho S358)抗体
英文名称Anti-MLKL (phospho S358) antibody
别名Mixed lineage kinase domain-like protein、hMLKL、MLKL
靶点符号MLKL
产品类型一抗

关键信息

宿主种属Rabbit
克隆Monoclonal
亚型IgG
不含载体蛋白No
反应种属Human
应用WB、Dot、IHC-P
免疫原The exact immunogen used to generate this antibody is proprietary information.
特异性Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the product protocols section. This file includes key technical notes of experience when using this product.
浓度0.855 mg/mL

反应性数据

应用应用代码物种验证状态推荐稀释备注
Dot BlotDotHuman保证反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPHuman已验证1/250 - 1/500Not Suitable for Mouse and Rat Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blotWBHuman已验证1/1000 - 1/2000We recommend using 1% SDS Hot lysis method to prepare cell lysates. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.
Dot BlotDotMouse不推荐1/1000
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPMouse不推荐1/250 - 1/500Not Suitable for Mouse and Rat Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blotWBMouse不推荐1/1000 - 1/2000We recommend using 1% SDS Hot lysis method to prepare cell lysates. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.
Dot BlotDotRat不推荐1/1000
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPRat不推荐1/250 - 1/500Not Suitable for Mouse and Rat Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blotWBRat不推荐1/1000 - 1/2000We recommend using 1% SDS Hot lysis method to prepare cell lysates. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here.
Dot BlotDotSynthetic peptide - Human已验证1/1000
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPSynthetic peptide - Human不推荐
Western blotWBSynthetic peptide - Human不推荐

储存与纯化

形式Liquid
纯化工艺Affinity purification Protein A
存储溶液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
推荐的短期储存时间1-2 weeks
储存信息Avoid freeze / thaw cycle
【仅供科学研究使用】

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