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βIII微管蛋白抗体(Anti-beta III Tubulin antibody - Neuronal Marker (RB9A37009277))

  • 货号:RB9A37009277
  • 价格:面议
  • 点击:2071
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产品介绍

产品描述

中文名称Anti-beta III Tubulin 抗体 - Neuronal Marker(RB9A37009277)
英文名称Anti-beta III Tubulin antibody - Neuronal Marker (RB9A37009277)
别名TUBB4、TUBB3、Tubulin beta-3 chain、Tubulin beta-4 chain、Tubulin beta-III
靶点符号TUBB3
产品类型一抗

关键信息

宿主种属Rabbit
克隆Polyclonal
亚型IgG
不含载体蛋白No
反应种属Mouse、Rat、Human、Common marmoset、Dogfish、Catshark
应用IHC-P、ICC/IF、WB、Flow Cyt (Intra)
免疫原The exact immunogen used to generate this antibody is proprietary information.
特异性The immunogen used for this product shares 75% homology with TUB (Tubby protein homolog, Uniprot: P50607). In western blot, we observe a specific band at ~55kDa which is not seen in KO cell lines. An additional band below this band of interest is seen at ~50kDa in both the WT and KO cells which could correspond to the protein TUB. Please note that cross-reactivity with this protein has not been confirmed experimentally. TUB is localized notably in high concentrations in the nucleoli of brain neurons with lower protein levels in the cytoplasm. Please, therefore, be aware that ICC experiments may need to be optimised. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. As an alternative antibody, we would recommend our recombinant rabbit monoclonal antibody RB9A37055656 which has been shown to specific in both WB and ICC using KO cells.
浓度0.2 mg/mL

反应性数据

应用应用代码物种验证状态推荐稀释备注
Flow Cytometry (Intracellular)FlowCytIntraCatshark保证反应0.01 µg for 10^6 CellsRB9A37007601 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed ( RB9A37060831 ) secondary antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFCatshark保证反应1 µg/mL
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPCatshark保证反应1/2000Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Western blotWBCatshark保证反应1 µg/mL
Flow Cytometry (Intracellular)FlowCytIntraCommon marmoset保证反应0.01 µg for 10^6 CellsRB9A37007601 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed ( RB9A37060831 ) secondary antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFCommon marmoset保证反应1 µg/mL
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPCommon marmoset保证反应1/2000Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Western blotWBCommon marmoset保证反应1 µg/mL
Flow Cytometry (Intracellular)FlowCytIntraDogfish保证反应0.01 µg for 10^6 CellsRB9A37007601 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed ( RB9A37060831 ) secondary antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFDogfish保证反应1 µg/mL
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPDogfish保证反应1/2000Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Western blotWBDogfish保证反应1 µg/mL
Flow Cytometry (Intracellular)FlowCytIntraHuman已验证0.01 µg for 10^6 CellsRB9A37007601 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed ( RB9A37060831 ) secondary antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFHuman保证反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPHuman已验证1/2000Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Western blotWBHuman已验证1 µg/mL
Flow Cytometry (Intracellular)FlowCytIntraMouse保证反应
Immunocytochemistry/ ImmunofluorescenceICCIFMouse保证反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPMouse保证反应
Western blotWBMouse已验证1 µg/mL
Flow Cytometry (Intracellular)FlowCytIntraPig预测反应
Immunocytochemistry/ ImmunofluorescenceICCIFPig预测反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPPig预测反应
Western blotWBPig预测反应
Flow Cytometry (Intracellular)FlowCytIntraRat保证反应
Immunocytochemistry/ ImmunofluorescenceICCIFRat已验证1 µg/mL
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPRat保证反应
Western blotWBRat已验证1 µg/mL
Flow Cytometry (Intracellular)FlowCytIntraRhesus monkey预测反应
Immunocytochemistry/ ImmunofluorescenceICCIFRhesus monkey预测反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPRhesus monkey预测反应
Western blotWBRhesus monkey预测反应

储存与纯化

形式Liquid
纯化工艺Affinity purification Immunogen
存储溶液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
推荐的短期储存时间1-2 weeks
储存信息Avoid freeze / thaw cycle
【仅供科学研究使用】

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