Anti-Human ETF1 Polyclonal Antibody|抗人ETF1多克隆抗体(靶标：人真核肽链释放因子1)
- 货号：RB9THD118014
- 价格：面议
- 点击：880
产品介绍
产品参数
| 形态 | Liquid |
|---|---|
| 宿主种属 | Rabbit |
| 克隆性 | Polyclonal |
| 抗体亚型 | IgG |
| 纯化方式 | Purified by antigen affinity column. |
| 内毒素水平 | Please contact with the lab for this information. |
| 应用 | ELISA, IHC, WB |
| 种属反应性 | Human, Mouse, Rat |
| 靶标 | ERF1, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Protein Cl1, RF1, SUP45L1, TB3-1, eRF1 |
| 免疫原 | E. coli - derived recombinant Human ETF1 (Met1-Ser257). |
| 储存缓冲液 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
| 产品使用说明 | ELISA:1:5000-1:20000;IHC:1:50-1:500;WB:1:500-1:2000 |
| 登录号 | P62495 |
| 背景信息 | Eukaryotic peptide chain release factor subunit 1 (ETF1) is a ~49 kDa protein. Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons. The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA. Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. 1. Song, H. et al. (2000) Cell 100, 311-21. PMID: 10676813 2. Alkalaeva, EZ. et al. (2006) Cell 125, 1125-36. PMID: 16777602 3. Feng, T. et al. (2014) Molecular cell 53, 645-54. PMID: 24486019 4. Brown, A. et al. (2015) Nature 524, 493-496. PMID: 26245381 5. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242 6. Oltion, K. et al. (2023) Cell 186, 346-362.e17. PMID: 36638793 7. Frolova, L. et al. (1994) Nature 372, 701-3. PMID: 7990965 8. Yamashita, A. et al. (2009) Genes & development 23, 1091-105. PMID: 19417104 9. Wang, X. et al. (2019) Cell 176, 625-635.e14. PMID: 30682371 |
| 备注 | For research use only |
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