Anti-Human ORF2p Polyclonal Antibody|抗人ORF2p多克隆抗体(靶标：人ORF2p蛋白)
- 货号：RB9THD130014
- 价格：面议
- 点击：263
产品介绍
产品参数
| 形态 | Liquid |
|---|---|
| 宿主种属 | Rabbit |
| 克隆性 | Polyclonal |
| 抗体亚型 | IgG |
| 纯化方式 | Purified by antigen affinity column. |
| 内毒素水平 | Please contact with the lab for this information. |
| 应用 | ELISA, IHC, WB |
| 种属反应性 | Human |
| 靶标 | EC:2.7.7.49, EC:3.1.21.-, Endonuclease, LINE-1 retrotransposable element ORF2 protein, ORF2p, Reverse transcriptase |
| 免疫原 | E. coli - derived recombinant Human ORF2p (Met1-Lys240). |
| 储存缓冲液 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
| 产品使用说明 | ELISA:1:5000-1:20000;IHC:1:50-1:500;WB:1:500-1:2000 |
| 登录号 | O00370 |
| 背景信息 | LINE-1 retrotransposable element ORF2 protein (ORF2p) is a ~149 kDa protein. Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Selectively binds and reversely transcribes RNA with a poly(A) tail consisting of at least 20 adenosines. Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to the sites of LINE-1 integration in the genome. Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition. 1. Thawani, A. et al. (2024) Nature 626, 186-193. PMID: 38096901 2. Baldwin, ET. et al. (2024) Nature 626, 194-206. PMID: 38096902 3. Dombroski, BA. et al. (1994) Molecular and cellular biology 14, 4485-92. PMID: 7516468 4. Sassaman, DM. et al. (1997) Nature genetics 16, 37-43. PMID: 9140393 5. Repanas, K. et al. (2007) Nucleic acids research 35, 4914-26. PMID: 17626046 6. Miller, I. et al. (2021) Nucleic acids research 49, 11350-11366. PMID: 34554261 7. Feng, Q. et al. (1996) Cell 87, 905-16. PMID: 8945517 |
| 备注 | For research use only |
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