抗牛冠状病毒抗体间接免疫荧光

货号：RB913702167
价格：面议
点击：1707

产品介绍

Anti-Bovine Coronavirus抗体描述：小鼠单克隆抗体靶向牛冠状病毒核蛋白(Nucleoprotein) 宿主/亚型：Mouse / IgG1 经测试应用：ICC/IF（免疫细胞化学/免疫荧光）种属反应性：Bovine Coronavirus 免疫原：纯化的牛冠状病毒全长天然核蛋白性能与储存形式：液体纯度：Protein A 纯化存储溶液：pH 7.2, 0.09% 叠氮化钠防腐剂, PBS 储存条件：短期1-2周可存4°C；长期分装后存-20°C或-80°C，避免反复冻融应用说明 ICC/IF：推荐起始浓度为 5μg/mL 实验流程：一抗使用浓度5μg/mL孵育 → FITC标记的二抗 (1μg/mL) 检测 → DAPI染核验证数据图阳性对照：MDBK细胞感染BCV Mebus株 → 细胞质内可见明亮的绿色荧光信号，定位准确阴性对照：未感染病毒的MDBK细胞 → 无荧光信号，背景干净靶点背景核蛋白(N蛋白)分子量约50kDa，负责包装病毒RNA基因组，在病毒复制中起关键作用，高度保守【仅供科学研究使用】

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