抗血小板内皮细胞黏附分子1抗体（Anti-PECAM-1/CD31 antibody (RB9A37059868)）
- 货号：RB9A37059868
- 价格：面议
- 点击：1809
产品介绍
产品描述
| 中文名称 | Anti-PECAM-1/CD31 抗体（RB9A37059868） |
|---|---|
| 英文名称 | Anti-PECAM-1/CD31 antibody (RB9A37059868) |
| 别名 | CD31、Platelet endothelial cell adhesion molecule、PECAM-1、EndoCAM、GPIIA'、PECA1、PECAM1 |
| 靶点符号 | PECAM-1 |
| 产品类型 | 一抗 |
关键信息
| 宿主种属 | Mouse |
|---|---|
| 克隆 | Monoclonal |
| 亚型 | IgG1 |
| 轻链类型 | kappa |
| 不含载体蛋白 | No |
| 反应种属 | Human |
| 应用 | IHC-P、ICC/IF、IHC-Fr、Flow Cyt、WB |
| 免疫原 | The exact immunogen used to generate this antibody is proprietary information. |
反应性数据
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| Flow Cytometry | FlowCyt | Cynomolgus monkey | 预测反应 | ||
| Immunocytochemistry/ Immunofluorescence | ICCIF | Cynomolgus monkey | 预测反应 | ||
| Immunohistochemistry (Frozen sections) | IHCFr | Cynomolgus monkey | 预测反应 | ||
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Cynomolgus monkey | 预测反应 | ||
| Western blot | WB | Cynomolgus monkey | 预测反应 | ||
| Flow Cytometry | FlowCyt | Human | 已验证 | 1/20 | |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Human | 已验证 | 1 µg/mL | Use 0.3M glycine to quench autofluorescence caused by aldehydes. |
| Immunohistochemistry (Frozen sections) | IHCFr | Human | 已验证 | 1 µg/mL | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 0.5 µg/mL | The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Western blot | WB | Human | 已验证 | 1/1000 | Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Hu Isoform 1-6: 79-83 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates ; Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate . |
储存与纯化
| 形式 | Liquid |
|---|---|
| 纯化工艺 | Affinity purification |
| 存储溶液 | Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine |
| 推荐的短期储存时间 | 1-2 weeks |
| 储存信息 | Avoid freeze / thaw cycle |
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