| 中文名称 | HRP Anti-GAPDH 抗体 - Loading Control |
|---|---|
| 英文名称 | HRP Anti-GAPDH antibody - Loading Control [Mouse] |
| 别名 | GAPD、CDABP0047、OK/SW-cl.12、GAPDH、Glyceraldehyde-3-phosphate dehydrogenase、Peptidyl-cysteine S-nitrosylase GAPDH |
| 靶点符号 | GAPDH |
| 产品类型 | 一抗 |
| 宿主种属 | Mouse |
|---|---|
| 克隆 | Monoclonal |
| 亚型 | IgG2b |
| 轻链类型 | kappa |
| 偶联物 | HRP |
| 不含载体蛋白 | No |
| 反应种属 | Mouse、Rat、Human |
| 应用 | WB |
| 免疫原 | The exact immunogen used to generate this antibody is proprietary information. |
| 浓度 | 0.5 mg/mL |
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| Western blot | WB | Chicken | 预测反应 | ||
| Western blot | WB | Chinese hamster | 预测反应 | ||
| Western blot | WB | Cow | 预测反应 | ||
| Western blot | WB | Dog | 预测反应 | ||
| Western blot | WB | Human | 已验证 | 1/1000 - 1/5000 | NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, RB9A37059859 ). This change in characteristics of the ab is due to a recent update to the production process. |
| Western blot | WB | Mouse | 已验证 | 1/1000 - 1/5000 | NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, RB9A37059859 ). This change in characteristics of the ab is due to a recent update to the production process. |
| Western blot | WB | Rabbit | 预测反应 | ||
| Western blot | WB | Rat | 已验证 | 1/1000 - 1/5000 | NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, RB9A37059859 ). This change in characteristics of the ab is due to a recent update to the production process. |
| Western blot | WB | Xenopus laevis | 预测反应 |
| 形式 | Liquid |
|---|---|
| 存储溶液 | Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA |
| 推荐的短期储存时间 | 1-2 weeks |
| 储存信息 | Avoid freeze / thaw cycle|Store in the dark |
| 纯度 | IgG fraction |