| 中文名称 | Anti-IL-6 抗体(RB9A37059727) |
|---|---|
| 英文名称 | Anti-IL-6 antibody [Mouse] |
| 别名 | IFNB2、IL6、Interleukin-6、IL-6、B-cell stimulatory factor 2、CTL differentiation factor、Hybridoma growth factor、Interferon beta-2、BSF-2、CDF、IFN-beta-2 |
| 靶点符号 | IL6 |
| 产品类型 | 一抗 |
| 宿主种属 | Mouse |
|---|---|
| 克隆 | Monoclonal |
| 亚型 | IgG2a |
| 不含载体蛋白 | No |
| 反应种属 | Rat、Human |
| 应用 | WB、sELISA、IHC-P |
| 免疫原 | Recombinant Full Length Protein corresponding to Human IL6.;P05231 |
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 1 µg/mL | Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
| Western blot | WB | Human | 保证反应 | ||
| Sandwich ELISA | sELISA | Human | 保证反应 | ||
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Rat | 保证反应 | ||
| Western blot | WB | Rat | 已验证 | To detect human IL-6 by Western blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-6 is 1.0-2.0 ng/lane, under reducing conditions and non-reducing conditions. | |
| Sandwich ELISA | sELISA | Rat | 保证反应 | ||
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Recombinant full length protein - Human | 不推荐 | ||
| Western blot | WB | Recombinant full length protein - Human | 不推荐 | ||
| Sandwich ELISA | sELISA | Recombinant full length protein - Human | 已验证 | 1-2 mg/mL | In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-6 when used with RB9A37034594 detection antibody at a concentration of approximately 0.10-0.20 μg/ml. |
| 形式 | Lyophilized |
|---|---|
| 纯化工艺 | Affinity purification Protein A |
| 推荐的短期储存时间 | 1-2 weeks |
| 复溶 | Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. |