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波形蛋白抗体(Anti-Vimentin antibody - Cytoskeleton Marker (RB9A37059654))

  • 货号:RB9A37059654
  • 价格:面议
  • 点击:2183
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产品介绍

产品描述

中文名称Anti-Vimentin 抗体 - Cytoskeleton Marker(RB9A37059654)
英文名称Anti-Vimentin antibody - Cytoskeleton Marker (RB9A37059654)
别名Vimentin、VIM
靶点符号VIM
产品类型一抗

关键信息

宿主种属Rabbit
克隆Monoclonal
亚型IgG
不含载体蛋白No
反应种属Mouse、Rat、Human、African green monkey
应用IHC-P、WB、mIHC、ICC/IF、Flow Cyt (Intra)
免疫原The exact immunogen used to generate this antibody is proprietary information.

反应性数据

应用应用代码物种验证状态推荐稀释备注
Flow Cytometry (Intracellular)FlowCytIntraAfrican green monkey保证反应
Immunocytochemistry/ ImmunofluorescenceICCIFAfrican green monkey保证反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPAfrican green monkey保证反应
Western blotWBAfrican green monkey已验证1/1000 - 1/5000
Multiplex immunohistochemistrymIHCAfrican green monkey保证反应
Flow Cytometry (Intracellular)FlowCytIntraCat预测反应
Immunocytochemistry/ ImmunofluorescenceICCIFCat预测反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPCat预测反应
Western blotWBCat预测反应
Multiplex immunohistochemistrymIHCCat预测反应
Flow Cytometry (Intracellular)FlowCytIntraHuman已验证1/50 - 1/500RB9A37007757 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFHuman已验证2 µg/mLThis product gave a positive signal in HeLa (VIM knockout HeLa cells were used as a negative control) fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPHuman已验证1/200 - 1/500Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blotWBHuman已验证1/1000 - 1/5000
Multiplex immunohistochemistrymIHCHuman已验证
Flow Cytometry (Intracellular)FlowCytIntraMouse保证反应
Immunocytochemistry/ ImmunofluorescenceICCIFMouse保证反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPMouse已验证1/200 - 1/500Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blotWBMouse已验证1/1000 - 1/5000
Multiplex immunohistochemistrymIHCMouse保证反应
Flow Cytometry (Intracellular)FlowCytIntraPig预测反应
Immunocytochemistry/ ImmunofluorescenceICCIFPig预测反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPPig预测反应
Western blotWBPig预测反应
Multiplex immunohistochemistrymIHCPig预测反应
Flow Cytometry (Intracellular)FlowCytIntraRat保证反应
Immunocytochemistry/ ImmunofluorescenceICCIFRat保证反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPRat已验证1/200 - 1/500Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blotWBRat已验证1/1000 - 1/5000
Multiplex immunohistochemistrymIHCRat保证反应
Flow Cytometry (Intracellular)FlowCytIntraRhesus monkey预测反应
Immunocytochemistry/ ImmunofluorescenceICCIFRhesus monkey预测反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPRhesus monkey预测反应
Western blotWBRhesus monkey预测反应
Multiplex immunohistochemistrymIHCRhesus monkey预测反应

储存与纯化

形式Liquid
纯化工艺Affinity purification Protein A
存储溶液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
推荐的短期储存时间1-2 weeks
储存信息Avoid freeze / thaw cycle
【仅供科学研究使用】

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