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抗基质金属蛋白酶2抗体(Anti-MMP2 antibody (RB9A37059646))

  • 货号:RB9A37059646
  • 价格:面议
  • 点击:1050
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产品介绍

产品描述

中文名称Anti-MMP2 抗体(RB9A37059646)
英文名称Anti-MMP2 antibody (RB9A37059646)
别名CLG4A、MMP2、72 kDa type IV collagenase、72 kDa gelatinase、Gelatinase A、Matrix metalloproteinase-2、TBE-1、MMP-2
靶点符号MMP2
产品类型一抗

关键信息

宿主种属Rabbit
克隆Monoclonal
亚型IgG
不含载体蛋白No
反应种属Mouse、Rat、Human
应用WB、Flow Cyt (Intra)、ICC/IF
免疫原The exact immunogen used to generate this antibody is proprietary information.
特异性In Western Blot, this product typically gives a weaker signal in some hepatocarcinoma like HepG2, MHCC97L (PMID: 33184263) et al. Please use recommended positive control when testing these cells.Compared with RB9A37059646, RB9A37009060 has higher sensitivity. We recommend RB9A37009060 as an alternative for testing MMP2 in western blot.This antibody shows low affinity in detecting mouse liver and HepG2 lysates which are positive for MMP2 reported by PMID 24096707 and 24297510.

反应性数据

应用应用代码物种验证状态推荐稀释备注
Flow Cytometry (Intracellular)FlowCytIntraHuman已验证1/400For unpurified, use 1/70. RB9A37007757 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFHuman已验证1/250
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPHuman不推荐
Western blotWBHuman已验证1/1000 - 1/5000For Lysate preparation protocol, please refer to the protocol here. Compared with RB9A37059646, RB9A37009060 has higher sensitivity. We recommend RB9A37009060 as an alternative for testing MMP2 in western blot.
Flow Cytometry (Intracellular)FlowCytIntraMouse保证反应
Immunocytochemistry/ ImmunofluorescenceICCIFMouse保证反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPMouse不推荐
Western blotWBMouse已验证1/1000 - 1/5000For Lysate preparation protocol, please refer to the protocol here. Compared with RB9A37059646, RB9A37009060 has higher sensitivity. We recommend RB9A37009060 as an alternative for testing MMP2 in western blot.
Flow Cytometry (Intracellular)FlowCytIntraRat保证反应
Immunocytochemistry/ ImmunofluorescenceICCIFRat保证反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPRat不推荐
Western blotWBRat已验证1/1000 - 1/5000For Lysate preparation protocol, please refer to the protocol here. Compared with RB9A37059646, RB9A37009060 has higher sensitivity. We recommend RB9A37009060 as an alternative for testing MMP2 in western blot.

储存与纯化

形式Liquid
纯化工艺Affinity purification Protein A
存储溶液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
推荐的短期储存时间1-2 weeks
储存信息Stable for 12 months at -20°C
【仅供科学研究使用】

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