抗S100A8抗体（Anti-S100A8 antibody (RB9A37059569)）
- 货号：RB9A37059569
- 价格：面议
- 点击：2020
产品介绍
产品描述
| 中文名称 | Anti-S100A8 抗体（RB9A37059569） |
|---|---|
| 英文名称 | Anti-S100A8 antibody (RB9A37059569) |
| 别名 | CAGA、CFAG、MRP8、S100A8、Protein S100-A8、Calgranulin-A、Calprotectin L1L subunit、Cystic fibrosis antigen、Leukocyte L1 complex light chain、Migration inhibitory factor-related protein 8、S100 calcium-binding protein A8、Urinary stone protein band A、MRP-8、p8 |
| 靶点符号 | S100A8 |
| 产品类型 | 一抗 |
关键信息
| 宿主种属 | Rabbit |
|---|---|
| 克隆 | Monoclonal |
| 亚型 | IgG |
| 不含载体蛋白 | No |
| 反应种属 | Mouse、Human |
| 应用 | WB、IP、IHC-P |
| 免疫原 | The exact immunogen used to generate this antibody is proprietary information. |
| 特异性 | S100A8 is abundantly expressed in neutrophils and inflammatory monocytes, but the expression would be downregulated when monocytes differentiate into macrophages and dendritic cells (PMID: 18809714, PMID: 1373330). Some customers have used this antibody successfully in western blot with mouse cell line lysates; however, our latest internal tests were unsuccessful and detection in mouse cell lysates cannot be guaranteed.For western blot experiments, it is strongly recommended to include appropriate positive controls, such as human HL 60 cell lysates or mouse spleen lysates, which are enriched for neutrophils and immature myeloid cells and consistently show robust S100A8 expression.For samples overexpressing S100A8, it is recommended to avoid placing the tag protein at the C-terminus of S100A8, as this may lead to negative results. |
反应性数据
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| Flow Cytometry | FlowCyt | Human | 不推荐 | 1/100 | RB9A37007757 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Human | 不推荐 | ||
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 1/500 - 1/1000 | For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
| Immunoprecipitation | IP | Human | 已验证 | 1/30 | For unpurified use at 1/20. |
| Western blot | WB | Human | 已验证 | 1/1000 | For unpurified use at 1/2000 - 1/10000. Some customers have used this antibody successfully in western blot with mouse cell line lysates; however, our latest internal tests were unsuccessful and detection in mouse cell lysates cannot be guaranteed. For western blot experiments, it is strongly recommended to include appropriate positive controls, such as human HL 60 cell lysates or mouse spleen lysates, which are enriched for neutrophils and immature myeloid cells and consistently show robust S100A8 expression. For samples overexpressing S100A8, it is recommended to avoid placing the tag protein at the C-terminus of S100A8, as this may lead to negative results. |
| Flow Cytometry | FlowCyt | Mouse | 不推荐 | 1/100 | RB9A37007757 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Mouse | 不推荐 | ||
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Mouse | 保证反应 | ||
| Immunoprecipitation | IP | Mouse | 保证反应 | ||
| Western blot | WB | Mouse | 已验证 | 1/1000 | For unpurified use at 1/2000 - 1/10000. Some customers have used this antibody successfully in western blot with mouse cell line lysates; however, our latest internal tests were unsuccessful and detection in mouse cell lysates cannot be guaranteed. For western blot experiments, it is strongly recommended to include appropriate positive controls, such as human HL 60 cell lysates or mouse spleen lysates, which are enriched for neutrophils and immature myeloid cells and consistently show robust S100A8 expression. For samples overexpressing S100A8, it is recommended to avoid placing the tag protein at the C-terminus of S100A8, as this may lead to negative results. |
储存与纯化
| 形式 | Liquid |
|---|---|
| 纯化工艺 | Affinity purification Protein A |
| 存储溶液 | pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA |
| 推荐的短期储存时间 | 1-2 weeks |
| 储存信息 | Avoid freeze / thaw cycle |
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