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抗核纤层蛋白B1抗体(Anti-Lamin B1 antibody - Nuclear Envelope Marker [Mouse])

  • 货号:RB9A37059324
  • 价格:面议
  • 点击:1141
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产品介绍

产品描述

中文名称Anti-Lamin B1 抗体 - Nuclear Envelope Marker(RB9A37059324)
英文名称Anti-Lamin B1 antibody - Nuclear Envelope Marker [Mouse]
别名Lamin-B1
靶点符号Lmnb1
产品类型一抗

关键信息

宿主种属Mouse
克隆Monoclonal
亚型IgG1
轻链类型kappa
不含载体蛋白No
反应种属Mouse、Human、Pig
应用IHC-Fr、Flow Cyt、ICC/IF
免疫原Native Full Length Protein corresponding to Rat Lmnb1.;P70615
特异性Reacts with an epitope located C-terminal of residue 231 in lamin B1. Reacts against lamin B1, does not cross react with lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. RB9A37059324 has been shown to react with HeLa and 3T3 cells in immunocytochemistry. Other cell/tissue types have not been tested.High levels of background are often observed when staining mouse tissue with a mouse antibody. We recommend RB9A37006799 as an alternative for mouse staining.
浓度1 mg/mL
表位C-terminal to residue 231.

反应性数据

应用应用代码物种验证状态推荐稀释备注
Flow CytometryFlowCytHuman已验证1/100 - 1/200RB9A37007391 - Mouse monoclonal , is suitable for use as an isotype control with this antibody.
Immunocytochemistry/ ImmunofluorescenceICCIFHuman已验证0.5 µg/mLFor example, if the product concentration is 1 mg/mL, the corresponding dilution required to achieve the recommended working concentration of 0.5 µg/mL would be 1:2000. Optimization of the ICC/IF protocol may be necessary depending on the sample used. Please note that the cells in our internal validation were fixed with 100% methanol.
Immunohistochemistry (Frozen sections)IHCFrHuman已验证
Flow CytometryFlowCytMouse保证反应
Immunocytochemistry/ ImmunofluorescenceICCIFMouse已验证0.5 µg/mLFor example, if the product concentration is 1 mg/mL, the corresponding dilution required to achieve the recommended working concentration of 0.5 µg/mL would be 1:2000. Optimization of the ICC/IF protocol may be necessary depending on the sample used. Please note that the cells in our internal validation were fixed with 100% methanol.
Immunohistochemistry (Frozen sections)IHCFrMouse保证反应
Flow CytometryFlowCytPig保证反应
Immunocytochemistry/ ImmunofluorescenceICCIFPig保证反应
Immunohistochemistry (Frozen sections)IHCFrPig已验证

储存与纯化

形式Liquid
纯化工艺Affinity purification Protein G
存储溶液Preservative: 0.09% Sodium azide Constituents: PBS
推荐的短期储存时间1-2 weeks
储存信息Avoid freeze / thaw cycle
【仅供科学研究使用】

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