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I型胶原蛋白抗体(Anti-Collagen I antibody - BSA and Azide free [Mouse])

  • 货号:RB9A37059131
  • 价格:面议
  • 点击:2236
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产品介绍

产品描述

中文名称Anti-Collagen I抗体 - BSA and Azide free
英文名称Anti-Collagen I antibody - BSA and Azide free [Mouse]
别名Collagen alpha-1(I) chain、Alpha-1 type I collagen、COL1A1
靶点符号COL1A1
产品类型一抗

关键信息

宿主种属Mouse
克隆Monoclonal
亚型IgG3
轻链类型kappa
不含载体蛋白Yes
反应种属Mouse、Human
应用WB、IHC-P、ELISA
免疫原Recombinant Fragment Protein within Human COL1A1 aa 1000-1150. The exact immunogen used to generate this antibody is proprietary information.;P02452
特异性We tested the antibody in mouse samples and, in our hands, it worked well in WB with NIH/3T3 cell line but not with other samples (tissue lysates). Compared with RB9A37010560, RB9A37034526 showed higher affinity. We recommend RB9A37034526 as an alternative for detecting mouse Collagen I in WB and IHC-P.

反应性数据

应用应用代码物种验证状态备注
ELISAELISAHuman保证反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPHuman已验证
Western blotWBHuman保证反应
ELISAELISAMouse保证反应
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPMouse不推荐
Western blotWBMouse已验证Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.
ELISAELISARecombinant fragment - Human已验证
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHCPRecombinant fragment - Human不推荐
Western blotWBRecombinant fragment - Human不推荐

储存与纯化

形式Liquid
存储溶液pH: 7.4 Constituents: PBS
推荐的短期储存时间1-2 weeks
储存信息Avoid freeze / thaw cycle
纯度Tissue culture supernatant
【仅供科学研究使用】

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