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小鼠E3泛素蛋白连接酶(CBLB)酶联免疫吸附检测试剂盒

  • 货号:ELK9290
  • 价格:¥2,800.00 元
  • 点击:2187

产品介绍

产品信息

英文名称Mouse CBLB(E3 ubiquitin-protein ligase CBLB) ELISA Kit
别名CBLB; Signal transduction protein CBL-B; SH3-binding protein CBL-B; Casitas B-lineage lymphoma proto-oncogene b; RING finger protein 56

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Mouse
检测类型Sandwich
灵敏度0.065 ng/mL
检测范围0.16-10 ng/mL
样本类型serum, plasma, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CBLB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CBLB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CBLB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CBLB in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q3TTA7

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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