小鼠核因子κB亚基p65亲和肽(NFκB p65)酶联免疫吸附检测试剂盒
- 货号：ELK9154
- 价格：￥2,800.00 元
- 点击：2014
产品介绍
产品信息
| 英文名称 | Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit |
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| 别名 | NF kappa B p65delta3; NFKB3 |
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规格与价格
| 48T 价格 | 1960 |
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| 96T 价格 | 2800 |
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| 96T*5 价格 | 11900 |
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检测参数
| 种属 | Mouse |
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| 检测类型 | Sandwich |
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| 灵敏度 | 12.2 pg/mL |
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| 检测范围 | 31.25-2000 pg/mL |
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| 样本类型 | serum, plasma, tissue homogenates |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Immunology |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt编号 | Q04207 |
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标准曲线
| 标准曲线图 |  |
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