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小鼠核因子κB亚基p65亲和肽(NFκB p65)酶联免疫吸附检测试剂盒

  • 货号:ELK9154
  • 价格:¥2,800.00 元
  • 点击:2425

产品介绍

产品信息

英文名称Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit
别名NF kappa B p65delta3; NFKB3

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Mouse
检测类型Sandwich
灵敏度12.2 pg/mL
检测范围31.25-2000 pg/mL
样本类型serum, plasma, tissue homogenates
实验时长3.5h

研究信息

研究领域Immunology
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q04207

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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