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斑马鱼白血病抑制因子(LIF)酶联免疫吸附检测试剂盒

  • 货号:ELK8514
  • 价格:¥2,800.00 元
  • 点击:1626

产品介绍

产品信息

英文名称Zebrafish LIF (leukemia inhibitory factor) ELISA Kit
别名CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Zebrafish
检测类型Sandwich
灵敏度6.4 pg/mL
检测范围15.63-1000 pg/mL
样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Cytokine;Tumor immunity;Infection immunity;Neuro science;Bone metabolism;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish LIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish LIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish LIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish LIF in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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