小鼠基质金属蛋白酶原-9(Pro-MMP-9)酶联免疫吸附检测试剂盒
- 货号：ELK8500
- 价格：￥2,800.00 元
- 点击：1808
产品介绍
产品信息
| 英文名称 | Mouse Pro-MMP-9(Pro-Matrix Metalloproteinase 9) ELISA Kit |
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| 别名 | Pro-MMP-9 |
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规格与价格
| 48T 价格 | 1960 |
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| 96T 价格 | 2800 |
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| 96T*5 价格 | 11900 |
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检测参数
| 种属 | Mouse |
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| 检测类型 | Sandwich |
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| 灵敏度 | 0.479 ng/mL |
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| 检测范围 | 0.79-50 ng/mL |
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| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids. |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Enzyme & Kinase;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology; |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-MMP-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Pro-MMP-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Pro-MMP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Pro-MMP-9 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
| 标准曲线图 |  |
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