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大鼠L-型电压依赖性钙离子通道α1D亚基(CACNa1D)酶联免疫吸附检测试剂盒

  • 货号:ELK8436
  • 价格:¥2,800.00 元
  • 点击:826

产品介绍

产品信息

英文名称Rat CACNa1D(Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit) ELISA Kit
别名CACH3; CACN4; CACNL1A2; CCHL1A2; Cav1.3; Voltage-gated calcium channel subunit alpha Cav1.3; Calcium channel, L type, alpha-1 polypeptide, isoform 2

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度0.108 ng/mL
检测范围0.32-20 ng/mL
样本类型Tissue homogenates, cell lysates and other biological fluids.
实验时长3.5h

研究信息

研究领域Signal transduction;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CACNa1D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CACNa1D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CACNa1D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CACNa1D in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P27732

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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