牛凝血因子Ⅱ(F2)酶联免疫吸附检测试剂盒

英文名称	Cattle F2(Coagulation Factor II) ELISA Kit
别名	FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2

研究领域	Signal transduction;Hematology;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle F2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle F2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P00735

标准曲线图

【仅供科学研究使用】

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