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猪胰岛素样生长因子结合蛋白4(IGFBP4)酶联免疫吸附检测试剂盒

  • 货号:ELK8240
  • 价格:¥2,800.00 元
  • 点击:2211

产品介绍

产品信息

英文名称Pig IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit
别名BP4; HT29-IGFBP; IBP4

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Pig
检测类型Sandwich
灵敏度0.65 ng/mL
检测范围1.57-100 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Apoptosis;Tumor immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IGFBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IGFBP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P24854

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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