大鼠肌肉生长抑制素(MSTN)微量上样酶联免疫吸附检测试剂盒

英文名称	Rat MSTN(Myostatin) Microsample ELISA Kit
别名	GDF8; Growth Differentiation Factor 8

种属	Rat
检测类型	Sandwich
灵敏度	0.254 ng/mL
检测范围	0.63-40 ng/mL
样本类型	serum, plasma and other biological fluids
实验时长	3.5h

研究领域	Cytokine;Metabolic pathway;Bone metabolism;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MSTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MSTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MSTN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	O35312

标准曲线图

【仅供科学研究使用】

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