环磷酸腺苷(cAMP)酶联免疫吸附检测试剂盒
- 货号：ELK8116
- 价格：￥2,400.00 元
- 点击：1346
产品介绍
产品信息
| 英文名称 | cAMP(Cyclic Adenosine Monophosphate) ELISA Kit |
|---|---|
| 别名 | c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate |
规格与价格
| 48T 价格 | 1680 |
|---|---|
| 96T 价格 | 2400 |
| 96T*5 价格 | 10200 |
检测参数
| 种属 | General |
|---|---|
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 92.2 pg/mL |
| 检测范围 | 312.5-20000 pg/mL |
| 样本类型 | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| 实验时长 | 2h |
研究信息
| 研究领域 | Signal transduction;Metabolic pathway; |
|---|---|
| 检测原理 | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cAMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve. |
标准曲线
| 标准曲线图 |  |
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